Taiwanese Journal of Obstetrics and Gynecology
Volume 46, Issue 2 , Pages 135-145, June 2007

The Interactions Between GPR30 and the Major Biomarkers in Infiltrating Ductal Carcinoma of the Breast in an Asian Population

  • Wen-Hung Kuo

      Affiliations

    • Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan
    • Angiogenesis Research Center, National Taiwan University, Taipei, Taiwan
    • ,
  • Li-Yun Chang

      Affiliations

    • Department of Obstetrics and Gynecology, National Taiwan University Hospital, Taipei, Taiwan
    • ,
  • Daisy Li-Yu Liu

      Affiliations

    • Biometrics Division, Department of Agronomy, National Taiwan University, Taipei, Taiwan
    • ,
  • Hsiao-Lin Hwa

      Affiliations

    • Department of Obstetrics and Gynecology, National Taiwan University Hospital, Taipei, Taiwan
    • ,
  • Jen-Jen Lin

      Affiliations

    • Department of Applied Statistics and Information Science, Ming Chuan University, Taipei, Taiwan
    • ,
  • Po-Huang Lee

      Affiliations

    • Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan
    • Angiogenesis Research Center, National Taiwan University, Taipei, Taiwan
    • ,
  • Chiung-Nien Chen

      Affiliations

    • Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan
    • Angiogenesis Research Center, National Taiwan University, Taipei, Taiwan
    • Division of Mechanics, Research Center for Applied Sciences, Academia Sinica, Taipei, Taiwan
    • ,
  • Huang-Chun Lien

      Affiliations

    • Department of Pathology, National Taiwan University Hospital, Taipei, Taiwan
    • ,
  • Ray-Hwang Yuan

      Affiliations

    • Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan
    • ,
  • Chia-Tung Shun

      Affiliations

    • Department of Pathology, National Taiwan University Hospital, Taipei, Taiwan
    • ,
  • King-Jen Chang

      Affiliations

    • Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan
    • Angiogenesis Research Center, National Taiwan University, Taipei, Taiwan
    • ,
  • Fon-Jou Hsieh

      Affiliations

    • Department of Obstetrics and Gynecology, National Taiwan University Hospital, Taipei, Taiwan
    • Graduate Institute of Clinical Research, National Taiwan University Hospital, Taipei, Taiwan
    • Department of Life Science, National Taiwan University, Taipei, Taiwan
    • Center of Systems Biology and Bioinformatics, National Taiwan University, Taipei, Taiwan
    • Corresponding Author InformationCorrespondence to: Dr Fon-Jou Hsieh, Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University Hospital, 7, Chung-Shan South Road, Taipei 100, Taiwan

Accepted 12 March 2007.

Summary 

Objective

G-protein-coupled receptor 30 (GPR30) has been reported to be a novel estrogen receptor a (ERa) in vitro. Therefore, the interactions among GPR30, ERa, progesterone receptor (PR) and human epidermal growth factor receptor-2 (HER-2/neu), and their prognostic utilities in the infiltrating ductal carcinoma (IDC) of the breast were evaluated.

Materials and Methods

Messenger RNA (mRNA) levels of GPR30, ERa, PR and HER-2/neu in the tumor samples of 118 Taiwanese IDC patients and 27 non-tumor mammary tissues were measured via quantitative polymerase chain reaction analyses. The correlations of GPR30 mRNA levels with clinical parameters, i.e. tumor/non-tumor, ERa, PR, HER-2/neu, age, lymph node metastasis, lymph–vascular invasion, grade, stage and patient survival, were assessed by using appropriate statistical analyses.

Results

GPR30 expression was observed to be lower in IDC (p < 0.001) than in non-tumor mammary tissues. Importantly, GPR30 mRNA level was positively correlated with that of ERa (p = 0.001) and PR (p = 0.001) but not correlated with that of HER-2/neu when they were analyzed as continuous variables. However, lower GPR30 was noticed in tumors with HER-2/neu protein overexpression. GPR30 expression was not correlated with age, lymph node metastasis, lymph–vascular invasion, grade and stage in IDC. GPR30 expression was not an independent prognostic factor for patient survival.

Conclusion

GPR30 expression is downregulated in IDC. GPR30 is preferentially co-expressed with ER and/or PR but is lowly expressed in HER-2/neu(+) tumors. The correlation of GPR30 expression with clinical parameters, including patient survival, was not evident in this cohort.

Key Words:  estrogen receptor α , G-protein-coupled receptor 30 , human epidermal growth factor receptor-2 , mRNA , progesterone receptor

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PII: S1028-4559(07)60007-2

doi:10.1016/S1028-4559(07)60007-2

Taiwanese Journal of Obstetrics and Gynecology
Volume 46, Issue 2 , Pages 135-145, June 2007

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