Taiwanese Journal of Obstetrics and Gynecology
Volume 46, Issue 2 , Pages 146-151 , June 2007

Comparison of Immunohistochemical and Fluorescence In Situ Hybridization Assessment for HER-2/neu Status in Taiwanese Breast Cancer Patients

  • Shou-Jen Kuo

      Affiliations

    • Department of Medical Research, National Taiwan University, and Hospital, Taipei, Taiwan
    • Department of Surgery, National Taiwan University, and Hospital, Taipei, Taiwan
    • ,
  • Boris Bao-Tyan Wang

      Affiliations

    • Department of Medical Research, National Taiwan University, and Hospital, Taipei, Taiwan
    • Department of Center for Medical Genetics, National Taiwan University, and Hospital, Taipei, Taiwan
    • ,
  • Cheng-Shyong Chang

      Affiliations

    • Department of Hematology-Oncology, National Taiwan University, and Hospital, Taipei, Taiwan
    • ,
  • Tze-Ho Chen

      Affiliations

    • Department of Medical Research, National Taiwan University, and Hospital, Taipei, Taiwan
    • Department of Obstetrics and Gynecology, National Taiwan University, and Hospital, Taipei, Taiwan
    • ,
  • Kun-Tu Yeh

      Affiliations

    • Department of Medical Research, National Taiwan University, and Hospital, Taipei, Taiwan
    • Department of Pathology, Changhua Christian Hospital, Changhua, Taipei, Taiwan
    • ,
  • Dong-Jay Lee

      Affiliations

    • Department of Medical Research, National Taiwan University, and Hospital, Taipei, Taiwan
    • Department of Center for Medical Genetics, National Taiwan University, and Hospital, Taipei, Taiwan
    • ,
  • Pao-Lun Yin

      Affiliations

    • Department of Center for Medical Genetics, National Taiwan University, and Hospital, Taipei, Taiwan
    • ,
  • Ming Chen

      Affiliations

    • Department of Medical Research, National Taiwan University, and Hospital, Taipei, Taiwan
    • Department of Center for Medical Genetics, National Taiwan University, and Hospital, Taipei, Taiwan
    • Department of Obstetrics and Gynecology, National Taiwan University, and Hospital, Taipei, Taiwan
    • Department of Obstetrics and Gynecology, National Taiwan University, and Hospital, Taipei, Taiwan
    • Department of Medical Genetics, National Taiwan University, and Hospital, Taipei, Taiwan
    • Corresponding Author InformationCorrespondence to: Dr Ming Chen, Center for Medical Genetics, Changhua Christian Hospital, Changhua 500, Taiwan

,Accepted 3 April 2007.

References 

  1. Slamon DJ , Leyland-Jones B , Shak S , et al.   Use of chemo-therapy plus a monoclonal antibody against HER2 for metastatic breast cancer that overexpresses HER2 . N Engl J Med . 2001;344:783–792
  2. Huang CS , Chen YC , Wang M , Lin JK , Chang KJ . HER-2/neu overexpression in Chinese breast cancers: correlation with other prognostic factors . J Formos Med Assoc . 1995;94:660–665
  3. Ross JS , Fletcher JA , Bloom KJ , et al.   HER-2/neu testing in breast cancer . Am J Clin Pathol . 2003;120(Suppl):S53–S71
  4. Dolan M , Snover D . Comparison of immunohistochemical and fluorescence in situ hybridization assessment of HER-2 status in routine practice . Am J Clin Pathol . 2005;123:766–770
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  9. Hou MF , Lin SB , Yuan SS , et al.   The clinical significance between activation of nuclear factor kappa B transcription factor and overexpression of HER-2/neu oncoprotein in Taiwanese patients with breast cancer . Clin Chim Acta . 2003;334:137–144
  10. Chen HH , Su WC , Guo HR , Chang TW , Lee WY . p53 and c-erbB-2 but not bcl-2 are predictive of metastasis-free survival in breast cancer patients receiving post-mastectomy adjuvant radiotherapy in Taiwan . Jpn J Clin Oncol . 2002;32:332–339
  11. Lan C, Liu JM, Liu TW, Hsu DH, Liang S, Chen JR, et al. erb-b2 amplification by fluorescence in situ hybridization in breast cancer specimens read as 2+ in immunohistochemical analysis . Am J Clin Pathol . 2005;124:97–102
  12. Pauletti G, Dandekar S, Rong H, Ramos L, Peng H, Seshadri R, et al. Assessment of methods for tissue-based detection of the HER-2/neu alteration in human breast cancer: A direct comparison of fluorescence in situ hybridization and immunohistochemistry . J Clin Oncol . 2000;18:3651–3664
  13. Lebeau A , Deimling D , Kaltz C , et al.   HER-2/neu analysis in archival tissue samples of human breast cancer: comparison of immunohistochemistry and fluorescence in situ hybridization . J Clin Oncol . 2001;19:354–363
  14. Horten B , Da Silva M , Thompson J . HER2 testing . CAP Today . 2001;15:8–9
  15. Tapia C , Schraml P , Simon R , et al.   HER2 analysis in breast cancer: reduced immunoreactivity in FISH non-informative cancer biopsies . Int J Oncol . 2004;25:1551–1557
  16. Vincent-Salomon A , MacGrogan G , Couturier J , et al.   Calibration of immunohistochemistry for assessment of HER2 in breast cancer: results of the French multicentre GEFPICS study . Histopathology . 2003;42:337–347
  17. Hicks DG , Yoder BJ , Pettay J , et al.   The incidence of topoisomerase II-alpha genomic alterations in adenocarcinoma of the breast and their relationship to human epidermal growth factor receptor-2 gene amplification: a fluorescence in situ hybridization study . Hum Pathol . 2005;36:348–356

PII: S1028-4559(07)60008-4

doi: 10.1016/S1028-4559(07)60008-4

Taiwanese Journal of Obstetrics and Gynecology
Volume 46, Issue 2 , Pages 146-151 , June 2007

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