Taiwanese Journal of Obstetrics and Gynecology
Volume 48, Issue 1 , Pages 65-68, March 2009

Long-chain Polyunsaturated Fatty Acids as Anti-HIV Supplementation During Breastfeeding

  • Kate Hsu

      Affiliations

    • Corresponding Author InformationCorrespondence to: Dr Kate Hsu, Department of Medical Research, Mackay Memorial Hospital, 45 Min-Sheng Road, Research Building, 616, Tamsui 251, Taiwan

Department of Medical Research, Mackay Memorial Hospital, Tamsui, Taiwan

Accepted 5 February 2009.

Article Outline

Summary 

Objective

Breastfeeding by human immunodeficiency virus (HIV)-positive mothers is an unavoidable practice in some very poor countries. It has been suggested that long-chain polyunsaturated fatty acids (LC-PUFAs) in breast milk, such as arachidonic acid, act as natural, protective ingredients against HIV transmission. The objective of this study was to identify the protective mechanism of LC-PUFAs in cells susceptible to HIV infection (e.g. human CD4 + T cells, HeLa cells).

Results

LC-PUFAs are bioactive molecules capable of activating the cellular protective machinery via modulation of endogenous background K + or KCNK channels. KCNK channel expression contributes significantly to the stability of the cell membrane potential. During HIV-1 infection, degradation of the KCNK channel is accelerated, and the cell membrane potential becomes pathologically depolarized. From studying functionally distinct KCNK mutants, we found that the degree of membrane potential depolarization was directly proportional to the release efficiency of HIV-1 virions. On the other hand, supplementation of KCNK channel modulators such as arachidonic acid (AA) and docosahexaenoic acid (DHA) at micromolar doses could restore hyperpolarization and stability of the cell membrane potential when endogenous KCNK channels are partially knocked down (mimicking the depolarized state of an HIV-1-infected cell).

Conclusion

The protective mechanism of LC-PUFAs against HIV spread involves stimulation of the endogenous KCNK channels. Our work suggests that supplementation with AA and DHA may be beneficial in reducing the risk of HIV-1 transmission, particularly during the period of breastfeeding.

Key Words:  HIV infection , K2P channels , KCNK channels , long-chain polyunsaturated fatty acids , transmission

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PII: S1028-4559(09)60038-3

doi:10.1016/S1028-4559(09)60038-3

Taiwanese Journal of Obstetrics and Gynecology
Volume 48, Issue 1 , Pages 65-68, March 2009

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