Taiwanese Journal of Obstetrics and Gynecology
Volume 44, Issue 3 , Pages 252-257, September 2005

Human Papillomavirus Testing (Hybrid Capture Ii) to Detect High-Grade Cervical intraepithelial Neoplasia in Women with Mildly Abnormal Papanicolaou Results

  • Hung-Sheng Chen

      Affiliations

    • Corresponding Author InformationCorrespondence to: Dr. Hung-Sheng Chen, Department of Obstetrics and Gynecology, Mackay Memorial Hospital, 92, Section 2, Chung-Shan North Road, Taipei, Taiwan
    • ,
  • Tsung-Hsien Su
  • Yang-Cheng Yang

      Affiliations

    • Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan
    • ,
  • Tao-Yeuan Wang

      Affiliations

    • Department of Pathology, Mackay Memorial Hospital, Taipei, Taiwan
    • ,
  • Yu-Wen Huang

      Affiliations

    • Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan

Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taipei, Taiwan

Received 7 March 2005; received in revised form 7 March 2005; accepted 8 March 2005.

Article Outline

Summary 

Objective

To evaluate the clinical value of human papillomavirus (HPV) DNA testing (Hybrid Capture II) in predicting which women with mild cervical cytologic abnormality (atypical squamous cells of undetermined significance [ASCUS] or low-grade squamous intraepithelial lesions [LSIL]) are most likely to have high-grade cervical intraepithelial neoplasia (CIN).

Materials and Methods

A total of 266 women with mildly abnormal Papanicolaou (Pap) smear results (160 with ASCUS and 106 with LSIL) concurrently underwent Hybrid Capture II testing, repeat Pap smear, and colposcopy-directed biopsies.

Results

Overall, the underlying prevalence of histologically confirmed CIN2/3 was 16.9% (27/160) in women with ASCUS and 29.2% (31/106) in those with LSIL. High-risk HPV DNA was detected in 55% and 88.7%, respectively. The sensitivity of CIN2/3 detection by HPV DNA was 96.3% (26/27) and 100% (31/31) among women with ASCUS and LSIL, respectively.

Conclusion

A positive baseline HPV test in women with ASCUS or LSIL is clinically equivalent, requiring colposcopic examination and denoting an approximately one-third chance of having CIN2/3. Among women with ASCUS, a negative HPV test strongly indicates that the patient is free of CIN2/3, which should minimize a patient's anxiety and unnecessary colposcopic examinations.

Key Words:  atypical squamous cells of undetermined significance , human papillomavirus testing , Hybrid

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PII: S1028-4559(09)60148-0

doi:10.1016/S1028-4559(09)60148-0

Taiwanese Journal of Obstetrics and Gynecology
Volume 44, Issue 3 , Pages 252-257, September 2005

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