Volume 44, Issue 4 , Pages 314-317, December 2005
Intracytoplasmic Sperm Injection: Technical Improvement
Article Outline
Summary
Successful fertilization using intracytoplasmic sperm injection (ICSI) was first reported in 1988 and the first successful ICSI pregnancy was in 1992. Since then, this technology has quickly become an indispensable technique in reproductive medicine, and its validity has been verified through several clinical studies over the past 10 years. After several technologic improvements, the rate of ICSI fertilization is comparable to that of conventional in vitro fertilization. In this article, we discuss how ICSI has improved fertilization and pregnancy rates.
Key Words: intracytoplasmic sperm injection
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References
- . A preclinical evaluation of pronuclear formation by microinjection of human spermatozoa into human oocytes . Fertil Steril . 1988;49:835–842
- . Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte . Lancet . 1992;340:17–18
- . 1998 results generated from the American Society for Reproductive Medicine/Society for Assisted Reproductive Technology Registry . Fertil Steril . 2002;77:18–31
- . Blastocyst formation in in vitro fertilization versus intracytoplasmic sperm injection cycles: influence of the fertilization procedure . Fertil Steril . 2005;83:1397–1403
- . Effects of different hyaluronidase concentrations and mechanical procedures for cumulus cell removal on the outcome of intracytoplasmic sperm injection . Hum Reprod . 1997;12:2246–2250
- . Aggressive sperm immobilization prior to intracytoplasmic sperm injection with immature spermatozoa improves fertilization and pregnancy rates . Hum Reprod . 1996;11:1023–1029
- . Behaviour of spermatozoa in human oocytes displaying no or one pronucleus after intracytoplasmic sperm injection . Hum Reprod . 1994;9:2139–2144
- . The position of the metaphase II spindle cannot be predicted by the location of the first polar body in the human oocyte . Hum Reprod . 2000;15:1372–1376
- . Orientation of the first polar body of the oocyte at 6 or 12 o'clock during ICSI does not affect clinical outcome . Hum Reprod . 2000;15:1580–1585
- . Sperm deposition site during ICSI affects fertilization and development . Fertil Steril . 2000;73:31–37
- Embryo development and chromosomal anomalies after ICSI: effect of the injection procedure . Hum Reprod . 2001;16:306–312
- Relationship between meiotic spindle location with regard to the polar body position and oocyte developmental potential after ICSI . Hum Reprod . 2003;18:1289–1293
- . The usefulness of a piezo-micromanipulator in intracytoplasmic sperm injection in humans . Hum Reprod . 1999;14:448–453
- . Cytoskeletal organization defects and abortive activation in human oocytes after IVF and ICSI failure . Mol Hum Reprod . 2000;6:510–516
- . Technical and physiological aspects associated with the lower fertilization following intra cytoplasmic sperm injection (ICSI) in human . Theriogenology . 1998;49:33–41
- A trial to restore defective human sperm centrosomal function . Hum Reprod . 2005;20:1933–1937
- Centrosomal function assessment in human sperm using heterologous ICSI with rabbit eggs: a new male factor infertility assay . Mol Reprod Dev . 2004;67:360–365
- . Ca2+ oscillations in the activation of the egg and development of the embryo in mammals . Int J Dev Biol . 1998;42:1–10
- . Recombinant phospholipase Czeta has high Ca2+ sensitivity and induces Ca2+ oscillations in mouse eggs . J Biol Chem . 2004;279:10408–10412
- . Mammalian fertilization: from sperm factor to phospholipase Czeta . Biol Cell . 2004;96:37–45
PII: S1028-4559(09)60163-7
doi:10.1016/S1028-4559(09)60163-7
© 2005 Taiwan Association of Obstetric & Gynecology. Published by Elsevier Inc. All rights reserved.
Volume 44, Issue 4 , Pages 314-317, December 2005
