Taiwanese Journal of Obstetrics and Gynecology
Volume 45, Issue 2 , Pages 95-99, June 2006

in Vitro Maturation of Human Oocytes

  • Yu-Hung Lin

      Affiliations

    • Department of Obstetrics and Gynecology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan
    • School of Medicine, Fu Jen Catholic University, Taipei, Taiwan
  • ,
  • Jiann-Loung Hwang

      Affiliations

    • Department of Obstetrics and Gynecology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan
    • Department of Obstetrics and Gynecology, Taipei Medical University, Taipei, Taiwan
    • Corresponding Author InformationCorrespondence to: Dr Jiann-Loung Hwang, Department of Obstetrics and Gynecology, Shin-Kong Wu Ho-Su Memorial Hospital, 95, Wen Chang Road, Shih Lin District, Taipei, Taiwan

Accepted 9 November 2005.

Article Outline

Summary

Immature oocyte retrieval followed by in vitro maturation (IVM) opens a new horizon for modern assisted reproductive technologies (ART). Recent studies in IVM make it a feasible alternative to in vitro fertilization. Antral follicle count is correlated with the pregnancy rate, so women with polycystic ovarian syndrome or polycystic ovaries are the best candidates for IVM. IVM can also be offered to women at risk of ovarian hyperstimulation syndrome or poor responders. From the available information, IVM is a safe procedure and does not increase congenital anomalies or pregnancy complications. Further research is necessary in order to apply this technique to other patients.

Key Words:  germinal vesicle , immature oocyte , in vitro maturation , polycystic ovarian syndrome

No full text is available. To read the body of this article, please view the PDF online.

 

Back to Article Outline

References 

  1. Durga Rao G , Chian RC , Son WS , Gilbert L , Tan SL . Fertility preservation in women undergoing cancer treatment . Lancet . 2004;363:1829–1830
  2. Tucker MJ , Wright G , Morton PC , et al.   Birth after cryopreservation of immature oocytes with subsequent in vitro maturation . Fertil Steril . 1998;70:578–579
  3. Cha KY , Koo JJ , Ko JJ , et al.   Pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program . Fertil Steril . 1991;55:109–113
  4. Hwang JL , Lin YH , Tsai YL . Pregnancy after immature oocyte donation and intracytoplasmic sperm injection . Fertil Steril . 1997;68:1139–1140
  5. Hwang JL , Lin YH , Tsai YL , et al.   Oocyte donation using immature oocytes from a normal ovulatory woman . Acta Obstet Gynecol Scand . 2002;81:274–275
  6. Cha KY , Chian RC . Maturation in vitro of immature human oocytes for clinical use . Hum Reprod Update . 1998;4:103–120
  7. Downs SM . Factors affecting the resumption of meiotic maturation in mammalian oocytes . Theriogenology . 1993;39:65–79
  8. Albertini DF , Carabatsos MJ . Comparative aspects of meiotic cell cycle control in mammals . J Mol Med . 1998;76:796–799
  9. Downs SM , Daniel SA , Eppig JJ . Induction of maturation in cumulus cell-enclosed mouse oocytes by follicle-stimulating hormone and epidermal growth factor: evidence for a positive stimulus of somatic cell origin . J Exp Zool . 1988;245:86–96
  10. De Sousa PA , Caveney A , Westhusin ME , et al.   Theriogenology . 1998;49:115–128
  11. Thibault C , Gerard M , Menezo Y . Preovulatory and ovulatory mechanisms in oocyte maturation . J Reprod Fertil . 1975;45:605–610
  12. Pincus G , Enzmann EV . The comparative behavior of mammalian eggs in vivo and in vitro . J Exp Med . 1935;62:665–675
  13. Edwards RG . Maturation in vitro of mouse, sheep, cow, pig, rhesus monkey and human ovarian oocytes . Nature . 1965;208:349–351
  14. Trounson A , Wood C , Kausche A . In vitro maturation and fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients . Fertil Steril . 1994;72:353–362
  15. Tsuji K , Sowa M , Nakano R . Relationship between human oocyte maturation and different follicular sizes . Biol Reprod . 1985;32:413–417
  16. Trounson A , Anderiesz C , Jones GM , et al.   Oocyte maturation . Hum Reprod . 1998;13(Suppl 3):52–62
  17. McGee E , Spears N , Minammi S , et al.   Preantral follicles in serum-free culture: suppression of apoptosis after activation of the cyclic guanosine 3_,5_-monophosphate pathway and stimulation of growth and differentiation by follicle-stimulating hormone . Endocrinology . 1997;138:2417–2424
  18. Schramm RD , Bavister BD . Follicle-stimulating hormone primig of rhesus monkeys enhances meiotic and developmental competence of oocyte matured in vitro . Biol Reprod . 1994;51:904–912
  19. Wynn P , Picton HM , Krapez JA , et al.   Pretreatment with follicle stimulating hormone promotes the numbers of human oocytes reaching metaphase II by in vitro maturation . Hum Reprod . 1998;13:3132–3138
  20. Mikkelsen AL , Lindenberg S . Benefit of FSH priming of women with PCOS to the in vitro maturation procedure and the outcome: a randomized prospective study . Reproduction . 2001;122:587–592
  21. Mikkelsen AL , Smith SD , Lindenberg S . In vitro maturation of human oocytes from regularly menstruating women may be successful without follicle stimulating hormone priming . Hum Reprod . 1999;14:1847–1851
  22. Chian RC , Gulekli B , Buckett WM , et al.   Priming with human chorionic gonadotropin before retrieval of immature oocytes in women with infertility due to the polycystic ovary syndrome . N Engl J Med . 1999;341:1624–1626
  23. Chian RC , Buckett WM , Tulandi T , et al.   Prospective randomized study of human chorionic gonadotrophin priming before immature oocyte retrieval from unstimulated women with polycystic ovarian syndrome . Hum Reprod . 2000;15:165–170
  24. Lin YH , Hwang JL , Huang LW , et al.   Combination of FSH priming and hCG priming for in vitro maturation of human oocytes . Hum Reprod . 2003;18:1632–1636
  25. Tan SL , Child TJ , Gulekli B . In vitro maturation and fertilization of oocytes from unstimulated ovaries: predicting the number of immature oocytes retrieved by early follicular phase ultrasonography . Am J Obstet Gynecol . 2002;186:684–689
  26. Child TJ , Abdul-Jalil AK , Gulekli B , Tan SL . In vitro maturation and fertilization of oocytes from unstimulated normal ovaries, polycystic ovaries, and women with polycystic ovary syndrome . Fertil Steril . 2001;76:936–942
  27. Child TJ , Phillips SJ , Abdul-Jalil AK , Bulekli B , Tan SL . A comparison of in vitro maturation and in vitro fertilization for women with polycystic ovaries . Obstet Gynecol . 2002;100:665–670
  28. Lim KS , Son WY , Yoon SH , Lim JH . IVM/F-ET in stimulated cycles for the prevention of OHSS . Fertil Steril . 2002;78(Suppl 3S):S11–S25
  29. Liu J , Lu G , Qian Y , Mao Y , Ding W . Pregnancies and births achieved from in vitro matured oocytes retrieved from poor responders undergoing stimulation in in vitro fertilization cycles . Fertil Steril . 2003;80:447–449
  30. Jaroudi KA , Hollanders JMG , Elnour AM , et al.   Embryo development and pregnancies from in vitro matured and fertilized oocytes . Hum Reprod . 1999;14:1749–1751
  31. Veeck L , Edwards J , Witmyer J , et al.   Maturation and fertilization of morphologically immature oocytes in a program of in vitro fertilization . Fertil Steril . 1983;39:594–602
  32. Nagy Z , Cecile J , Liu J , et al.   Pregnancy and birth after intracytoplasmic sperm injection of in vitro matured germinal vesicle stage oocytes: case report . Fertil Steril . 1996;65:1047–1050
  33. Jaroudi KA , Hollanders JMG , Sieck U , et al.   Pregnancy after transfer of embryos which were generated from in vitro matured oocytes . Hum Reprod . 1997;12:857–859
  34. Chian RC , Tan SL . Maturational and developmental competence of cumulus-free immature human oocytes derived from stimulated and intracytoplasmic sperm injection cycles . Reprod BioMed Online . 2002;5:125–132
  35. Kim BK , Lee SC , Kim KJ , et al.   In vitro maturation, fertilization, and development of human germinal vesicle oocytes collected from stimulated cycles . Fertil Steril . 2000;74:1153–1158
  36. Nogueira D , Staessen C , van de Velde H , et al.   Nuclear status and cytogenetics of embryos derived from in vitro matured oocytes . Fertil Steril . 2000;74:295–298
  37. Chian RC , Buckett WM , Abdul Jalil AK , et al.   Natural-cycle in vitro fertilization combined with in vitro maturation of immature oocytes is a potential approach in infertility treatment . Fertil Steril . 2004;82:1675–1678
  38. Cha KY , Chung HM , Lee DR , et al.   Obstetric outcome of patients with polycystic ovary syndrome treated by in vitro maturation and in vitro fertilization-embryo transfer . Fertil Steril . 2005;83:1461–1465
  39. Mikkelsen AL . Strategies in human in vitro maturation and their clinical outcome . Reprod BioMed Online . 2005;20:593–599
  40. Le Du A , Kadoch IJ , Bourcigaux N , et al.   In vitro oocyte maturation for the treatment of infertility associated with polycystic ovarian syndrome: the French experience . Hum Reprod . 2005;20:420–424

PII: S1028-4559(09)60204-7

doi:10.1016/S1028-4559(09)60204-7

Taiwanese Journal of Obstetrics and Gynecology
Volume 45, Issue 2 , Pages 95-99, June 2006