Taiwanese Journal of Obstetrics and Gynecology
Volume 48, Issue 2 , Pages 96-107, June 2009

Differential Expression of NUDT9 at Different Phases of The Menstrual Cycle and in Different Components of Normal and Neoplastic Human Endometrium

  • Ling-Yun Chen

      Affiliations

    • Institute of Biochemistry and Biotechnology, School of Medicine, Chung-Shan Medical University, Taichung, Taiwan
    • ,
  • Tze-Ho Chen

      Affiliations

    • Department of Obstetrics and Gynecology, Changhua Christian Hospital, Changhua, Taiwan
    • Co-first author
    • ,
  • Pao-Ying Wen

      Affiliations

    • Institute of Biochemistry and Biotechnology, School of Medicine, Chung-Shan Medical University, Taichung, Taiwan
    • Department of Genomic Medicine, Changhua Christian Hospital, Changhua, Taiwan
    • ,
  • Chia-Hung Chou

      Affiliations

    • Department of Oncology, National Taiwan University, College of Medicine, and Hospital, Taipei, Taiwan
    • ,
  • Tsung-Ho Ying

      Affiliations

    • Institute of Biochemistry and Biotechnology, School of Medicine, Chung-Shan Medical University, Taichung, Taiwan
    • Department of Obstetrics and Gynecology, Chung-Shan Medical University Hospital, Taichung, Taiwan
    • ,
  • Shun-Ping Chang

      Affiliations

    • Department of Medical Research, Changhua Christian Hospital, Changhua, Taiwan
    • ,
  • Gwo-Chin Ma

      Affiliations

    • Institute of Biochemistry and Biotechnology, School of Medicine, Chung-Shan Medical University, Taichung, Taiwan
    • Department of Genomic Medicine, Changhua Christian Hospital, Changhua, Taiwan
    • Department of Medical Research, Changhua Christian Hospital, Changhua, Taiwan
    • ,
  • Ming Chen

      Affiliations

    • Department of Obstetrics and Gynecology, Changhua Christian Hospital, Changhua, Taiwan
    • Department of Genomic Medicine, Changhua Christian Hospital, Changhua, Taiwan
    • Department of Medical Research, Changhua Christian Hospital, Changhua, Taiwan
    • Department of Obstetrics and Gynecology, College of Medicine and Hospital, Taipei, Taiwan
    • Department of Medical Genetics National Taiwan University, College of Medicine and Hospital, Taipei, Taiwan
    • Corresponding Author InformationCorrespondence to: Dr Ming Chen, Department of Genomic Medicine, Changhua Christian Hospital, Changhua, Taiwan

Accepted 16 February 2009.

Article Outline

Summary 

Objective

The human endometrium manifests different and distinct morphologies and physiologies during the different phases of the menstrual cycle. We aimed to determine which candidate genes demonstrate differential expression patterns in the endometrium during different phases of the menstrual cycle.

Materials and Methods

Using differential display reverse transcription polymerase chain reaction to compare day 5 and day 18 human glandular endometrium obtained by laser capture microdissection, we identified a specific gene, NUDT9 (nucleoside diphosphate-linked moiety X motif 9). NUDT9 is known to function as a highly specific adenosine diphosphate ribose pyrophosphatase and has been mapped to chromosome 4q22.1. It gives rise to two alternatively spliced messenger RNAs, NUDT9α and NUDT9β, encoding a member of the Nudix hydrolase family. In this study, we purified NUDT9 protein and produced an antibody, which we then used for immunohistochemical studies.

Results

Using this anti-NUDT9 antibody, we successfully demonstrated that NUDT9 protein was differentially expressed in endometrial glandular cells at different phases of the menstrual cycle. NUDT9 was also found to be expressed more prominently in the epithelial glandular component than in the stromal component of human endometrial carcinomas.

Conclusion

We suggest that NUDT9 may be involved in the regulation of the menstrual cycle and may be related to the proliferation of glandular cells in the human endometrium.

Key Words:  ADP-ribose pyrophosphatase , endometrial carcinoma , endometrium , Nudix , NUDT9

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PII: S1028-4559(09)60266-7

doi:10.1016/S1028-4559(09)60266-7

Taiwanese Journal of Obstetrics and Gynecology
Volume 48, Issue 2 , Pages 96-107, June 2009

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