Taiwanese Journal of Obstetrics and Gynecology
Volume 48, Issue 4 , Pages 370-374, December 2009

The Relationship Between Human Papillomavirus and Epstein-Barr Virus Infections in Relation to Age Of Patients With Cervical Adenocarcinoma

  • Hei-Yu Lau

      Affiliations

    • Section of Gynecologic Oncology, Department of Obstetrics and Gynecology, Taipei Veteran General Hospital, National Yang-Ming University, Taipei, Taiwan
    • ,
  • Nae-Fang Twu

      Affiliations

    • Section of Gynecologic Oncology, Department of Obstetrics and Gynecology, Taipei Veteran General Hospital, National Yang-Ming University, Taipei, Taiwan
    • Co-first author
    • ,
  • Paul Chih-Hsueh Chen

      Affiliations

    • Department of Pathology, Taipei Veterans General Hospital, Taipei, Taiwan
    • ,
  • Chiung-Ru Lai

      Affiliations

    • Department of Pathology, Taipei Veterans General Hospital, Taipei, Taiwan
    • ,
  • Chi-Mou Juang

      Affiliations

    • Section of Gynecologic Oncology, Department of Obstetrics and Gynecology, Taipei Veteran General Hospital, National Yang-Ming University, Taipei, Taiwan
    • ,
  • Ming-Shyen Yen

      Affiliations

    • Section of Gynecologic Oncology, Department of Obstetrics and Gynecology, Taipei Veteran General Hospital, National Yang-Ming University, Taipei, Taiwan
    • ,
  • Kuan-Chong Chao

      Affiliations

    • Section of Gynecologic Oncology, Department of Obstetrics and Gynecology, Taipei Veteran General Hospital, National Yang-Ming University, Taipei, Taiwan
    • Corresponding Author InformationCorrespondence to: Dr Kuan-Chong Chao, Department of Gynecology and Obstetrics, Taipei Veterans General Hospital, 201, Section 2, Shih-Pai Road, Taipei 112, Taiwan

Accepted 11 June 2009.

Article Outline

Summary 

Objective

To examine the relationship between human papillomavirus (HPV) and Epstein-Barr virus (EBV) infections in relation to age of patients with cervical adenocarcinoma.

Materials and Methods

Thirty samples of human cervical adenocarcinoma tissue were collected from the surgical pathology archive at Taipei Veterans General Hospital from 1996 to 2008. All samples were examined for EBV, HPV-16 and HPV-18 E6 DNA by conventional and real-time quantitative polymerase chain reaction assays.

Results

HPV-16 DNA was detected in 10 cases (33.3%), HPV-18 DNA in 12 cases (40%), and EBV DNA in three cases (10%); there were negative findings in seven cases (23.3%). EBV combined with HPV-16 or HPV-18 was also detected in one case each. No link could be demonstrated between HPV and EBV in endocervical lesions. When 20 patients £ 45 years old were compared with 10 patients > 45 years old, HPV-18 E6 DNA was detected in 45% vs. 30% (9/20 vs. 3/10), HPV-16 E6 DNA in 40% vs. 20% (8/20 vs. 2/10), EBV DNA in 10% vs. 10% (2/20 vs. 1/10), and no virus DNA was detected in 10% vs. 50% (2/20 vs. 5/10). HPV and EBV were significantly more common in younger women (p < 0.001).

Conclusion

HPV-18 plays a major role in adenocarcinomas at any age. A high prevalence of HPV DNA is significantly associated with cervical adenocarcinoma, especially in younger women. The results do not support a role for EBV in cervical adenocarcinogenesis or any relationship between EBV and HPV infection in adenocarcinoma.

Key Words:  cervical adenocarcinoma , Epstein-Barr virus , HPV-16 , HPV-18

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References 

  1. Vizcaino AP, Moreno V, Bosch FX, Munoz N, Barros-Dios XM, Borras J, et al. International trends in the incidence of cervical cancer, II: squamous-cell carcinoma . Int J Cancer . 2000;86:429–435
  2. Vizcaino AP , Moreno V , Bosch FX , Munoz N , Barros-Dios XM , Parkin DM . International trends in the incidence of cervical cancer, I: adenocarcinoma and adenosquamous cell carcinomas . Int J Cancer . 1998;75:536–545
  3. Bulk S , Visser O , Rozendaal L , Verheijen RH , Meijer CJ . Cervical cancer in the Netherlands 1989–1998: decrease of squamous cell carcinoma in older women, increase of adeno-carcinoma in younger women . Int J Cancer . 2005;113:1005–1009
  4. Pirog EC , Kleter B , Olgac S , et al.   Prevalence of human papillomavirus DNA in different histological subtypes of cervical adenocarcinoma . Am J Pathol . 2000;157:1055–1062
  5. Schiffman MH , Bauer HM , Hoover RN , et al.   Epidemiologic evidence showing that human papillomavirus infection causes most cervical intraepithelial neoplasia . J Natl Cancer Inst . 1993;85:958–964
  6. Walboomers JM , Jacobs MV , Manos MM , et al.   Human papillomavirus is a necessary cause of invasive cervical cancer worldwide . J Pathol . 1999;189:12–19
  7. zur Hausen H . Human papillomavirus in the pathogenesis of anogenital cancer . Virology . 1991;184:9–13
  8. Bosch FX , Manos MM , Munoz N , et al.   Prevalence of human papillomavirus in cervical cancer: a worldwide perspective . J Natl Cancer Inst . 1995;87:796–802
  9. Lorincz AT , Reid R , Jenson AB , Greenberg MD , Lancaster W , Kurman RJ . Human papillomavirus infection of the cervix: relative risk associations of 15 common anogenital types . Obstet Gynecol . 1992;79:328–337
  10. Castellsague X , Diaz M , de Sanjose S , et al.   Worldwide human papillomavirus etiology of cervical adenocarcinoma and its cofactors: implications for screening and prevention . J Natl Cancer Inst . 2006;98:303–315
  11. Hilton DA , Brown LJ , Pringle JH , Nandha H . Absence of Epstein-Barr virus in carcinoma of the cervix . Cancer . 1993;72:1946–1948
  12. van den Brule AJ , Walboomers JM , Meijer CJ . Epstein-Barr virus infection as co-factor in cervical carcinogenesis? . J Pathol . 1995;176:219–220
  13. Payne S , Kernohan NM , Walker F . Absence of in situ hybridization evidence for latent-or lytic-phase Epstein-Barr virus infection of preinvasive squamous lesions of the cervix . J Pathol . 1995;176:221–226
  14. Weinberg E , Hoisington S , Eastman AY , Rice DK , Malfetano J , Ross JS . Uterine cervical lymphoepithelial-like carcinoma: absence of Epstein-Barr virus genomes . Am J Clin Pathol . 1993;99:195–199
  15. Wong KY , Collins RJ , Srivastava G . Epstein-Barr virus in carcinoma of the cervix . Int J Gynecol Pathol . 1993;12:224–227
  16. Tase T , Sato S , Wada Y , Yajima A , Okagaki T . Prevalence of human papillomavirus type 18 DNA in adenocarcinoma and adenosquamous carcinoma of the uterine cervix occurring in Japan . Tohoku J Exp Med . 1988;156:47–53
  17. Peters RK , Chao A , Mack TM , Thomas D , Bernstein L , Henderson BE . Increased frequency of adenocarcinoma of the uterine cervix in young women in Los Angeles County . J Nat Cancer Inst . 1986;76:423–428
  18. Schwartz SM , Daling JR , Shera KA , et al.   Human papilloma-virus and prognosis of invasive cervical cancer: a population-based study . J Clin Oncol . 2001;19:1906–1915
  19. Werness BA , Levine AJ , Howley PM . Association of human papillomavirus types 16 and 18 E6 proteins with p53 . Science . 1990;248:76–79
  20. Singh P , Ilancheran A , Ratnam SS , Kim LT , O'Reilly AP . Cervical adenocarcinoma in women with nasopharyngeal carcinoma . Cancer . 1989;64:1152–1155

PII: S1028-4559(09)60325-9

doi:10.1016/S1028-4559(09)60325-9

Taiwanese Journal of Obstetrics and Gynecology
Volume 48, Issue 4 , Pages 370-374, December 2009

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